Welcome to the METABARPARK Project website. The project aims to apply the new high throughput sequencing technologies to characterize benthic communities in the Spanish Marine National Parks using environmental DNA (metabarcoding). Our main objective is to characterize the hidden biodiversity which is usually overlooked by traditional methods. We will also try to assess the community impacts of invasive seaweeds.

Friday, December 29, 2017

Results of the study of invasive algae published in Marine Pollution Bulletin

We are happy to report that the results concerning the invaded vs non-invaded communities sampled in 2015 have now been published in the international journal Marine Pollution Bulletin (MPB 127: 54-66, 2018).

Elsevier kindly provides a 50 days free access (starting 21 Dec) to this article at https://authors.elsevier.com/a/1WG02,ashmKxI
After this period you will need a subscription to be able to download it

In this paper we report on the effect that invasive algae have at the community level, targeting mainly the smaller size components (small macrobenthos and meiobenthos), using the 18S and the COI genes and the fractionation procedure and bioinformatic pipeline developed in our project. Here goes the abstract:

We analysed with multigene (18S and COI) metabarcoding the effects of the proliferation of invasive seaweeds on rocky littoral communities in two Spanish Marine Protected Areas. The invasive algae studied were Caulerpa cylindracea, Lophocladia lallemandii and Asparagopsis armata. They are canopy-forming, landscape-dominant seaweeds, and we were interested in their effects on the underlying communities of meiobenthos and macrobenthos, separated in two size fractions through sieving. A new semiquantitative treatment of metabarcoding data is introduced. The results for both markers showed that the presence of the invasive seaweed had a significant effect on the understory communities for Lophocladia lallemandii and Asparagopsis armata but not for Caulerpa cylindracea. Likewise, changes in MOTU richness and diversity with invasion status varied in magnitude and direction depending on the alga considered. Our results showed that metabarcoding allows monitoring of the less conspicuous, but not least important, effects of the presence of dominant invasive seaweeds.

Images of the communities analysed, with (left) and without (right) invasive algae. A,B: Cc (Caulerpa cylindracea) communities. C,D: Ll (Lophocladia lallemandii) communities. E,F: Aa (Asparagopsis armata) communities. Pictures: (A) from Eneko Aspillaga; (B,F) from the authors; (C,D) from Pol Capdevila; (E) from Enric Ballesteros

Results from Metabarpark samplings published in PeerJ Preprints

While the article is being peer-reviewed, we have published the manuscript in Pre-print format in the repository PeerJ Preprints, where it is freely available at https://peerj.com/preprints/3429/


Wangensteen OS, Palacín C, Guardiola M, Turon X (2017) PeerJ Preprints 5:e3429v1

We report in this manuscript the astonishing diversity found in communities from Islas Cies and Cabrera. We address several technical aspects, compare the two genes studied (18S and COI), and perform ecological analyses. Here goes the abstract:

We developed a metabarcoding method for biodiversity characterization of structurally complex natural marine hard-bottom communities. Novel primer sets for two different molecular markers: the “Leray fragment” of mitochondrial cytochrome c oxidase, COI, and the V7 region of ribosomal RNA 18S were used to analyse eight different marine shallow benthic communities from two National Parks in Spain (one in the Atlantic Ocean and another in the Mediterranean Sea). Samples were sieved into three size fractions from where DNA was extracted separately. Bayesian clustering was used for delimiting molecular operational taxonomic units (MOTUs) and custom reference databases were constructed for taxonomic assignment. We found unexpectedly high values for MOTU richness, suggesting that these communities host a large amount of yet undescribed eukaryotic biodiversity. Significant gaps are still found in sequence reference databases, which currently prevent the complete taxonomic assignation of the detected sequences. Nevertheless, over 90% (in abundance) of the sequenced reads could be successfully assigned to phylum or lower taxonomical level. This identification rate might be significantly improved in the future, as reference databases are updated. Our results show that marine metabarcoding, currently applied mostly to plankton or sediments, can be adapted to structurally complex hard bottom samples, and emerges as a robust, fast, objective and affordable method for comprehensively characterizing the diversity of marine benthic communities dominated by macroscopic seaweeds and colonial or modular sessile metazoans, allowing for standardized biomonitoring of these ecologically important communities. The new universal primers for COI can potentially be used for biodiversity assessment with high taxonomic resolution in a wide array of marine, terrestrial or freshwater eukaryotic communities.

Percent of MOTUs found for the main categories of organisms in the three fractions (A, B, C) studied with 18S (left) and COI (right) in all communities studied

Tuesday, July 11, 2017

Metabarpark at the Pint of Science festival in Blanes

The Pint of Science festival aims to deliver interesting talks on the latest science research in an accessible format to the public – mainly across bars and pubs (preferably with a pint of beer in hands). It is an International festival being held annually over three days at the same time in hundreds of cities across the world. Blanes, the host city of the CEAB, has participated for the first time in this year’s Pint of Science festival. The scientists of the CEAB gave nine talks three evenings (15-17 May 2017).

Xavier Turon participated in the first day of the festival, presenting the opening talk at a nice bar (Casal la Cooperativa) on the topic: Biodiversity 2.0: genetics and computers to study the living organisms. His talk introduced the topic of the ongoing sixth extinction caused by human pressures, and how computer science and genetics can help mitigating these effects. In particular, how we should complete biodiversity inventories as a first step towards conservation. The examples presented drew mainly from results obtained in Metabarpark.

It was an enjoyable and challenging experience to try to explain in lay terms some complex analysis we do in our research. The audience was very interested and asked many relevant questions after the talk.

The banner of the festival in Blanes

Monday, March 13, 2017

Metabarpark at the meeting "Developing new genetic tools for bioassessment of aquatic ecosystems in Europe"

Metabarpark was present at the meeting on metabarcoding held in Essen (Germany) in March 7th-9th 2017. This was the kick-off conference of the European Union COST action DNAqua-Net (http://dnaqua.net/).

This project aims at integrating a group of researchers across disciplines with the task to identify gold-standard genomic tools and novel eco-genomic indices and metrics for routine application for biodiversity assessments and biomonitoring of European water bodies. It also envisages training and dissemination activities. The objectives of Metabarpark are fully compatible with the goals of DNAqua-Net and we wanted to be counted in and contribute.

Xavier Turon is one of the Spanish members of DNAqua-Net and attended this conference, where ca. 200 researchers gathered together to present results, discuss hot topics, and delineate the way forward for achieving a wide acceptance by management bodies of genetic methods for biodiversity assessment in the European context.

Xavier presented the communication "Under the canopy: community-wide effects of invasive algae in marine protected areas revealed by metabarcoding", by Xavier Turon, Owen Wangensteen, Emma Cebrian and Creu Palacín. In this contribution the main results concerning the effects of three invasive algae (Caulerpa cylindracea and Lophocladia lallemandii in Cabrera, and Asparagopsis armata in the Atlantic) on the benthic communities were exposed. We have also drafted and sent a manuscript for publication with these results. This contribution is also the first result of the collaboration between the National Park projects Metabarpark and Corclim.

The logo of DNAqua-Net

A heatmap representation of the samples of communities with and without Asparagopsis armata from Cíes Islands, separated by invasion status (green: non-invaded, red: invaded) and fraction (blue: macrobenthos; turquoise: meiobenthos)

Friday, November 18, 2016

Metabarpark at the meeting "Jornadas de Investigación en la Red de Parques Nacionales 2016”

On October, 18 to 20, 2016 the meeting “Jornadas de Investigación en la Red de Parques Nacionales 2016” was held in the National Park of Tablas de Daimiel in Ciudad Real. This meeting gathered together staff from the OAPN central services, from the different National Parks, members of the Scientific Committee of the OAPN, and researchers that are developing projects in the National Park Network. The results of 13 projects corresponding to the 2013 call were presented in public talks, and there were also technical talks about programs currently underway in the Parks Network.

Xavier Turon gave a talk about the Metabarpark project, presenting the main findings and highlighting the potential of the genetic information for mid- and long- term biomonitoring of the communities.

This meeting has been a very good occasion for sharing experiences and discussing with colleagues and Park managers. Among the attendants were the staff from the Atlantic Islands Park (Pepín, Mercedes, Vicente), and many other acquaintances. It was such a pleasure to meet them all! I cannot resist mentioning also the fine gastronomy we enjoyed and the nice visit to the Tablas de Daimiel National Park that we did.

Family foto of the attendants to the meeting

Sunday, September 25, 2016

METABARPARK at the XIX Iberian Marine Biology Symposium

From 5th to 9th September the XIX Iberian Marine Biology Symposium was held in Porto (Portugal). It was organized by the CIIMAR - Interdisciplinary Centre of Marine and Environmental Research -, and gathered together ca. 180 scientists from Spain, Portugal, and some latin-american countries.

The webpage of the symposium is available http://siebmxixciimar.wixsite.com/business-conferenc-1

The Iberian meetings have been organized since 1978, and are an excellent opportunity for researchers in the Iberian zone to meet, exchange ideas, and present ongoing projects and results.

Three members of Metabarpark (Owen Wangensteen, Creu Palacín, and Xavier Turon) attended the meeting. They presented the oral contribution "Wangensteen, Guardiola, Palacín, Turon: Molecular biodiversity assessment of marine hard-bottom communities from Spanish National Parks by metabarcoding of COI and 18S" where a summary of the main findings of the Metabarpark project was given. The presentation raised a lot of interest among the audience, as shown in the questions posed after the talk and the many interactions that followed during the meeting.

Xavier Turon also presented a plenary conference, entitled "Twenty-five years of genetic studies of the Iberian benthos: retrospect and prospect". Needless to say, metabarcoding was among the main techniques highlighted during this talk.

The nice logo of the XIX SIEBM
We had a good and fun time in Porto, we made interesting contacts for future metabarcoding studies. We are looking forward to the next Iberian Meeting, to be held in Faro in 2018!

Sunday, April 10, 2016

Metabarcoding reveals significant differences in communities with and without invasive seaweeds

While the data corresponding to the 2015 collecting trips are being analysed, we would like to share the first results concerning the communities sampled with and without invasive algae. In the Cíes Island, we compared the photophilous community with Cystoseira nodicaulis (natural assemblage) with equivalent communities with the introduced Asparagopsis armata. In Cabrera, we sampled in the same walls photophilous natural assemblages as well as communities dominated by Lophocladia lallemandii, while at deeper waters we sampled the natural sciaphilous community and Caulerpa cylindracea-dominated patches.

We identified with the gene 18S 2937 MOTUs in these communities. Our statistical analyses showed clear qualitative and quantitative differences between assemblages with and without the invasive seaweeds. While this was expected for the bigger size fractions, even for the smallest fraction (i.e., organisms between 1 mm and 65 microns) the differences were significant. This rich component of the biodiversity cannot be efficiently assessed with conventional methods, while genetic techniques have allowed its quantification and analysis.

Thus, metabarcoding revealed community shifts affecting even the small-sized meio-organisms (animals, algae, and protists) at very reduced spatial scales (tens of meters). The presence of invasive algae has therefore profound effects in community structure at all levels. This is one of the several interesting outcomes we are finding with our data, more soon!
This spatial representation (Multidimensional Scaling) allows the visualization of the differences between the sampled communities linked to the presence of invasive seaweeds