Welcome to the METABARPARK Project website. The project aims to apply the new high throughput sequencing technologies to characterize benthic communities in the Spanish Marine National Parks using environmental DNA (metabarcoding). Our main objective is to characterize the hidden biodiversity which is usually overlooked by traditional methods. We will also try to assess the community impacts of invasive seaweeds.

Wednesday, January 20, 2016

Working with the data


We processed the samples, extracted the DNA and amplified the barcoding genes for the second sampling (2015) performed at each site. We received the sequences in November (for the COI gene) and December (for the 18S gene). So we have now all genetic data of the samplings done in 2014 and 2015 in Cíes Islands and the Cabrera Archipelago.

In the last months we have been busy working with the bioinformatic analyses of such a huge amount of data. We had to adapt and improve existing pipelines, write code ourselves, and check different approaches at each step of the process.

Now we are drafting our first manuscript for publication with the results of the first samplings with biodiversity assessment and community comparisons within and between the Parks. We have also assembled a large dataset with all sequences obtained in 2014 and 2015 that is currently being analysed to assess temporal changes in the community structure in both Parks. We hope to come up with interesting results soon!


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